In a domestic five-line ship lock project, the dimensions and operational parameters of each ship lock vary significantly.There are a large number of anchorages with different functions, and the ship navigation routes near the anchorages and locks are intertwined.Under the complex conditions, there is a lack of proper methods to analyze the operation rules of five-line ship lock.This article analyzes the operation rules of the five-line ship lock from two dimensions：the priority rules for ship entering and leaving the locks and the lock arrangement rules.Based on the simulation model of multi-line ship locks, a simulation modeling method considering ship navigation and introducing channel network initialization algorithm and channel navigation judgment algorithm is proposed.The results show that compared with the rule of “priority for incoming ships”, the passing capacity of the rule of “priority for outgoing ships” is about 20% higher.The adoption of “Lock No.1 is limited to ships with a width of more than 12 m, Lock No.2 is limited to ships with a width of less than 15m, and Locks No.3, 4, and 5 are limited to ships with a width of more than 10m” is slightly better than other scheduling rules.